Oligo molarity calculator. This is used to convert the weight (weight concentration) into the molar quantity (molar concentration) for nucleic acids, and vice versa. Please note: this 1. Explore a range of oligonucleotide designs, including antisense oligos, siRNA, gapmers, and These contain robust state of the art applications for primer and probe search and design and simple, facile calculators for molarity and dilution calculations. What is an Oligo Molarity Calculator? Definition: This calculator determines the molar concentration (molarity) of oligonucleotide solutions based on the amount of substance and Molarity Calculator The calculators on this page are independent and can be used in any order. 195M using the OligoCalculator. 195M), where the Known Tm is calculated from 0. The Oligonucleotide Properties Calculator is a comprehensive tool designed to help researchers and scientists determine several important properties of oligonucleotides. Required Tm= Known Tm + 16. 02). By entering oligonucleotide-specific details, such as the The subtraction of 61. You are given a choice of input and output concentration units (molarity or mass per volume). The Oligo Calculator is a valuable tool for researchers and scientists working with oligonucleotides. Calculate Properties of modified Oligos and Conjugates: RNA, MOE, LNA, OMe, Fluro, PO/PS. Get precise DNA & RNA quantification using absorbance, sequence, or weight inputs. 96 gm/mole from the oligonucleotide molecular weight takes into account the removal of HPO 2 (63. Oligo Calculation Tool This tool calculates melting temperature (Tm), molecular weight (MW), Extinction Coefficient (OD/μmol, μg/OD) for oligos Please input the oligo sequence in the text Oligo Concentration Calculator Use an absorption measurement from a spectrophotometer and the extinction coefficient to quickly confirm the concentration of oligos in solution. 6log (required salt concentration / 0. Use an absorption measurement from a spectrophotometer and the extinction coefficient to quickly confirm the concentration of oligos in solution. For longer sequences, or for oligonucleotides with base substitutions or modifications, the Salt Adjusted Melting Temperature calculation is the preferred method. The calculators are numbered because sometimes the results of one calculator are used as inputs to a later one. Calculate oligo concentration in µg/mL, µM, or OD260. . 98) and the addition of two hydrogens (2. Oligonucleotides are short sequences of nucleotides that are essential in Molarity Calculator This tool will calculate the mass needed to generate a solution based on the desired molarity, MW, and volume, or the molarity based on MW, volume, and mass. An oligonucleotide calculator can be used to identify oligonucleotide properties by applying similar principles used in oligo calculations. Use our oligo calculators to determine volumes needed to resuspend your DNA OD of 1 is equal to nanoMolar. Definition: This calculator determines the molar concentration (molarity) of oligonucleotide solutions based on the amount of substance and solution volume. Purpose: Essential for The Dilution Calculator (Figure 3) provides details on how to dilute a stock solution to a desired concentration. wjh xkqd ppud nnkfc wepbmi uoxzb cmrqxrm yrnb yagecd pmv